1、

The gene expression of a-actin mRNA were determined by using the quantitative reverse transcription PCR technology.

利用半定量反转录聚合酶链式反应(QRT-PCR)法测定骨骼肌α-actin基因表达.

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2、

Methods: Mainly with methods of in situ reverse transcription polymerase chain reaction ( In situ RT-PCR ).

方法以原位逆转录聚合酶链式反应 ( Insitureverse transcriptionpolymerasechainreaction,InsituRT -PCR ) 为主要研究方法.

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3、

G proteins mRNA expressions were detected by the method of reverse transcription polymearse chain reaction ( RT-PCR ).

橱)逆转录聚合酶链反应 ( RT—PCR ) 方法研究大鼠心肌Gsa蛋白、Gia蛋白mRNA的表达.

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5、

Then the expression of ATF was analyzed by means of reverse transcription polymerase chain reaction ( RT-PCR )

用反转录PCR ( RT-PCR ) 检测转染细胞ATF基因的表达.

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6、

The expressions of Slit 2 were tested by reverse transcription polymerase chain reaction ( RT-PCR ) and immunohistochemical measurement.

分别通过逆转录聚合酶链反应法 ( RT -PCR ) 和免疫组织化学方法检测Slit2基因的表达水平及部位.

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8、

TLR 4 mRNA in KCs was determined by the reverse transcription polymerase chain reaction ( RT-PCR )

用逆转录多聚酶联反应 ( RT -PCR ) 测定KCs中TLR4mRNA的表达.

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9、

Methods Reverse transcription-polymerase chain reaction ( RT-PCR ), and immunohistochemical method were used.

方法采用反转录 - 聚合酶链反应 ( RT -PCR ) 法和免疫组织化学方法.

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10、

The proto-oncogene c-fos mRNA expression was assessed using reverse transcription polymerase chain reaction ( RT-PCR ).

用逆转录聚合酶链式反应 ( RT -PCR ) 检测心肌细胞原癌基因c-fosmRNA的表达.

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11、

The gene expression of ET-1、 ETAR 、 ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response ( RT-PCR ).

采用半定量逆转录多聚酶链反应 ( RT -PCR ) 检测局部内皮素系统ET -1 、ETAR、ETBR及ECE的基因表达.

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12、

Expression of aquaporin 1 in renal tissue was observed by using real reverse transcription polymerase chain reaction.

实时逆转录聚合酶链式反应检测肾脏组织中水通道蛋白1表达.

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13、

Methods: ( 1) Reverse transcription-polymerase chain reaction ( RT-PCR) was use to obtain the TR gene complementary DNA ( cDNA).

方法:(1)进行逆转录-聚合酶链式反应(RT PCR),获得TR基因cDNA;

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14、

TGF β 1 mature peptide gene was amplified by reverse transcription polymerase chain reaction ( RT-PCR) using RNA LA PCR~ ( TM) Kit ( TaKaRa Co.)

使用Takara公司RNA LA PCR~(TM)逆转录试剂盒扩增TGF-β1成熟肽基因。

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15、

Preparation of esophageal carcinoma cDNA probe labeled with digoxigenin by reverse transcription reaction

逆转录法制备地高辛标记的食管癌cDNA探针

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16、

METHODS The traditional rat two kidney one clip ( 2K1C) renal hypertension model was modified and the expression of AT 1a mRNA, AT 1b mRNA was examined by quantitative reverse transcription polymerase chain reaction ( QRT PCR).

方法改进传统的两肾一夹肾性高血压大鼠实验模型制作方法,采用定量反转录聚合酶链式反应(QRT PCR)方法对AT1a mRNA、AT1b mRNA进行定量。

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17、

The expression of cardiac MLC-2 in 12 week-endurance trained rats was investigated by quantitative reverse transcription polymerize chain reaction technique ( QRT-PCR), using the 18S-RNA of rat as a internal refernce.

以大鼠18S-RNA为内参照,用定量反转录聚合酶链式反应(QRT-PCR)技术对对20只经过12周耐力训练的SD大鼠心肌中MLC-2基因的表达进行了研究。

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